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Fig. 3 | Lipids in Health and Disease

Fig. 3

From: FTY720 inhibited proinflammatory cytokine release and osteoclastogenesis induced by Aggregatibacter actinomycetemcomitans

Fig. 3

FTY720 suppressed osteoclastogenesis in bone marrow-derived pre-osteoclasts treated with or without A. actinomycetemcomitans (Aa) stimulation. a Bone marrow (BM) cells were treated as described in Methods. b Representative images show TRAP-stained cells with or without A. actinomycetemcomitans stimulation. Pictures were taken at 100× magnification. c Number of TRAP+ multinucleated (more than 3 nuclei) osteoclasts/well (96-well) were quantified. d Total areas for osteoclasts/image were quantified. e Cell viability was tested in bone marrow-derived pre-osteoclasts treated with vehicle or FTY720 (2 μM) for 24 h. Data are expressed as mean ± SEM (n = 4, ***p < 0.001)

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Lipids in Health and Disease

ISSN: 1476-511X