Table 2 Primer sequences used in real-time PCR analysis and ChIP assay
From: Lipopolysaccharide significantly influences the hepatic triglyceride metabolism in growing pigs
Target gene | Sequence (F: forward, R: reverse) | GenBank access | Applications |
|---|---|---|---|
TLR2 | F: GACACCGCCATCCTCATTCT | NC_010450 | PCR |
| Â | R: CTTCCCGCTGCGTCTCAT | Â | Â |
TLR4 | F: TCTACATCAAGTGCCCCTAC | NR_024169.1 | PCR |
| Â | R: TAAATTCTCCCAAAACCAAC | Â | Â |
TNF-α | F:CCACGCTCTTCTGCCTACTGC | JF831365.1 | PCR |
| Â | R:TCGGCTTTGACATTGGCTACAA | Â | Â |
NF-κB | F:GGGGACTACGACCTGAATGC | EU399817.1 | PCR |
| Â | R:CACGGTTGTCAAAGATGGG | Â | Â |
IL-1α | F:TACTGACTATGGCTACCAA | NM_214029.1 | PCR |
| Â | R:ATTCCAGCTGCTATTGTG | Â | Â |
ACC-1 | F:GGCCATCAAGGACTTCAACC | NM_001114269.1 | PCR |
| Â | R:ACGATGTAAGCGCCGAACTT | Â | Â |
FAS | F: GTCCTGCTGAAGCCTAACTC | EF589048 | PCR |
| Â | R: TCCTTGGAACCGTCTGTG | Â | Â |
SCD-1 | F: CCCAGCCGTCAAAGAGAA | NM_213781 | PCR |
| Â | R: CGATGGCGTAACGAAGAAA | Â | Â |
PPAR-γ | F: GCCCTTCACCACTGTTGATT | NM_138711 | PCR |
| Â | R: GAGTTGGAAGGCTCTTCGTG | Â | Â |
C/EBP-β | F: GACAAGCACAGCGACGAGTA | NM_001199889 | PCR |
| Â | R: AGCTGCTCCACCTTCTTCTG | Â | Â |
HSL | F: ACCCTCGGCTGTCAACTTCTT | AY686758 | PCR |
| Â | R: TCCTCCTTGGTGCTAATCTCGT | Â | Â |
ATGL | F: ACCTGTCCAACCTGCTGC | EF583921 | PCR |
| Â | R: GCCTGTCTGCTCCTTTATCCA | Â | Â |
CPT-1α | F:ACAACGAGGTCTTCCGAT | NM_001129805.1 | PCR |
| Â | R:AACGCAAAACCACCAAACCC | Â | Â |
ND1 | F: TCCTACTGGCCGTAGCATTCCT | KF888634.1 | PCR |
| Â | R: TTGAGGATGTGGCTGGTCGTAG | Â | Â |
ND2 | F: ATCGGAGGGTGAGGAGGGCTAA | KF888634.1 | PCR |
| Â | R: GTTGTGGTTGCTGAGCTGTGGA | Â | Â |
ND3 | F: AGCACGCCTCCCATTCTCAAT | KF888634.1 | PCR |
| Â | R: TGCTAGGCTTGCTGCTAGTAGG | Â | Â |
ND4 | F: TCGCCTATTCATCAGTAAGTCA | KF888634.1 | PCR |
| Â | R: GGATTATGGTTCGGCTGTGTA | Â | Â |
ND4L | F: GATCGCCCTTGCAGGGTTACTT | KF888634.1 | PCR |
| Â | R: CTAGTGCAGCTTCGCAGGCT | Â | Â |
ND5 | F: CGGATGAGAAGGCGTAGGAA | KF888634.1 | PCR |
| Â | R: GCGGTTGTATAGGATTGCTTGT | Â | Â |
ND6 | F: ACTGCTATGGCTACTGAGATGT | KF888634.1 | PCR |
| Â | R: CTTCCTCTTCCTTCAACGCATA | Â | Â |
COX1 | F: TGGTGCCTGAGCAGGAATAGTG | KF888634.1 | PCR |
| Â | R: ATCATCGCCAAGTAGGGTTCCG | Â | Â |
COX2 | F: GCTTCCAAGACGCCACTTCAC | KF888634.1 | PCR |
| Â | R: TGGGCATCCATTGTGCTAGTGT | Â | Â |
COX3 | F: GGCTACAGGGTTTCACGGGTTG | KF888634.1 | PCR |
| Â | R: TCAGTATCAGGCTGCGGCTTCA | Â | Â |
ATP6 | F: ACTCATTCACACCCACCACACA | KF888634.1 | PCR |
| Â | R: CCTGCTGTAATGTTGGCTGTCA | Â | Â |
ATP8 | F: TGCCACAACTAGATACATCC | KF888634.1 | PCR |
| Â | R: GCTTGCTGGGTATGAGTAG | Â | Â |
CYTB | F: CTGAGGAGCTACGGTCATCACA | KF888634.1 | PCR |
| Â | R: GCTGCGAGGGCGGTAATGAT | Â | Â |
PPIA | F: TCCTCCTTGGTGCTAATCTCGT | NM_214353.1 | PCR |
| Â | R: TGATCTTCTTGCTGGTCTT | Â | Â |
ACC-1_promoter | F: ACAGCATACGGAGTTCCTGG | Â | ChIP |
| Â | R: TGTCTTCTGCAACAATGGGA | Â | Â |