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Table 3 The changes in COPs contents (mg) in cholesterol with or without unsaturated fatty acids during heating at 100 °C

From: Monitoring the formation of cholesterol oxidation products in model systems using response surface methodology

Treat Hour 7β-OH*** 20α-OH*** 25-OH*** Triol*** α-epoxide*** 7-keto*** Total amount of COPs
C 1 n.d.d n.d.f 0.07 ± 0.00bc n.d.d 0.02 ± 0.01ghi 0.03 ± 0.00bc 0.11 ± 0.002ef
  3 0.08 ± 0.01d 0.11 ± 0.01e 0.05 ± 0.01bcd n.d.d 0.02 ± 0.00ghi 0.05 ± 0.00b 0.31 ± 0.03ef
  5 0.01 ± 0.00d n.d.f 0.01 ± 0.00f n.d.d n.d.i n.d.c 0.01 ± 0.001f
  10 n.d.d 0.02 ± 0.00f 0.01 ± 0.00ef n.d.d n.d.i n.d.c 0.03 ± 0.01f
CL1 1 1.47 ± 0.18b 0.21 ± 0.03c 0.06 ± 0.01bc n.d.d 0.02 ± 0.00fgh n.d.c 1.76 ± 0.22b
(1:20) 3 n.d.d 0.02 ± 0.00f 0.003 ± 0.00f n.d.d 0.13 ± 0.01b n.d.c 0.15 ± 0.01ef
  5 n.d.d 0.03 ± 0.01f 0.01 ± 0.00f n.d.d 0.16 ± 0.02a n.d.c 0.19 ± 0.03ef
  10 n.d.d 0.26 ± 0.08b 0.06 ± 0.01bc n.d.d 0.03 ± 0.01efgh 0.35 ± 0.09a 0.69 ± 0.18d
CL2 1 1.29 ± 0.08b 0.17 ± 0.01d 0.08 ± 0.01b 0.02 ± 0.00a 0.03 ± 0.00defg 0.06 ± 0.00b 1.64 ± 0.10b
(1:100) 3 n.d.d 0.002 ± 0.00f 0.16 ± 0.07a 0.01 ± 0.00b 0.05 ± 0.01cd n.d.c 0.22 ± 0.09ef
  5 0.01 ± 0.00d 0.01 ± 0.00f 0.004 ± 0.00f 0.01 ± 0.00c n.d.i n.d.c 0.04 ± 0.004f
  10 n.d.d 0.01 ± 0.00f 0.002 ± 0.00f n.d.d n.d.i n.d.c 0.01 ± 0.002f
CO1 1 0.66 ± 0.03c 0.29 ± 002b 0.04 ± 0.00cde n.d.d 0.01 ± 0.00hi n.d.c 1.01 ± 0.05c
(1:20) 3 n.d.d 0.0003 ± 0.00f 0.01 ± 0.00f n.d.d 0.04 ± 0.00def n.d.c 0.05 ± 0.003f
  5 n.d.d 0.02 ± 0.00f 0.01 ± 0.00f n.d.d 0.12 ± 0.02b n.d.c 0.15 ± 0.03ef
  10 n.d.d 0.36 ± 0.05a 0.02 ± 0.00def n.d.d 0.03 ± 0.00defg n.d.c 0.42 ± 0.06de
CO2 1 3.79 ± 0.63a 0.18 ± 0.02cd 0.06 ± 0.01bc 0.02 ± 0.00a 0.03 ± 0.00def 0.05 ± 0.00b 4.12 ± 0.67a
(1:100) 3 n.d.d 0.004 ± 0.00f 0.006 ± 0.00f n.d.d 0.06 ± 0.03c n.d.c 0.07 ± 0.03f
  5 n.d.d 0.01 ± 0.00f 0.003 ± 0.00f n.d.d 0.04 ± 0.00de n.d.c 0.05 ± 0.01f
  10 n.d.d 0.01 ± 0.00f n.d.f n.d.d n.d.i n.d.c 0.01 ± 0.001f
  1. C cholesterol (50 mg), L linoleic acid, O: oleic acid, CL 1 cholesterol (50 mg) : linoleic acid (1 g) = 1 : 20, CL 2 cholesterol (50 mg) : linoleic acid (5 g) =1 : 100, CO 1 cholesterol (50 mg) : oleic acid (1 g) = 1 : 20, CO 2 cholesterol (50 mg) : oleic acid (5 g) = 1 : 100
  2. 7β-OH 7β-hydroxycholesterol, 20α-OH 7α-hydroxy-cholesterol, 25-OH 25-hydroxycholesterol, Triol Cholestanetriol, α-epoxide 5,6α-epoxycholesterol, 7-keto: 7-ketocholesterol
  3. *** P < 0.001. n.d not detected
  4. a–i Means ± SE with different superscript in the same column differ significantly