Skip to main content
Fig. 3 | Lipids in Health and Disease

Fig. 3

From: Palmitate induces cisternal ER expansion via the activation of XBP-1/CCTα-mediated phospholipid accumulation in RAW 264.7 cells

Fig. 3

Palmitate induces ER stress in RAW 264.7 cells. Cells were treated with palmitate at the indicated concentration for 24 h (a and b) and with 400 μM palmitate for the indicated times (c and d). The phosphorylation of PERK, and the expression of BiP and CHOP were determined by western blot analysis (a and c). Total RNA was extracted and reverse transcribed to cDNA. Full-length and spliced XBP-1 cDNAs were amplified by PCR with mouse XBP-1 primers. Gapdh cDNA was included as an internal loading control (b and d)

Back to article page

Lipids in Health and Disease

ISSN: 1476-511X