Table 1 Primers and fluorescent probes of human apoM, human GAPDH, rat apoM and ratβ-actin
Human apoM | |
|---|---|
forward primer | 5’ TGC CCC GGA AAT GGA TCT A 3’ |
reverse primer | 5’ CAG GGC GGC CTT CAG TT 3’ |
probe | 5’ FAM-CAC CTG ACT GAA GGG AGC ACA GAT CTC A-TAMRA 3’ |
Human GAPDH | |
forward primer | 5’GGA AGG TGA AGG TCG GAG TC 3’ |
reverse prime | 5’CGT TCT CAG CCT TGA CGG T 3’ |
probe | 5’FAM- TTT GGT CGT ATT GGG CGC CTG -TAMRA3’ |
Rat apoM | |
forward primer | 5’ ACAAAGAGACCCCAGAGCCC 3’ |
reverse primer | 5’ TCCATGGTGGGAGCCG 3’ |
probe | 5’ FAM- ACCTGGGCCTGTGGTACTTTATTGCTGG -TAMRA 3’ |
Rat β-actin | |
forward primer | 5’ GCCACTGCCGCATCCTCT 3’ |
reverse prime | 5’ CTGGAAGAGAGCCTCGGGG 3’ |
probe | 5’FAM-AGCTGCCTGACGGTCAGGTCATCACTATC -TAMRA3’ |