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Fig. 3 | Lipids in Health and Disease

Fig. 3

From: Mevalonate inhibits acid sphingomyelinase activity, increases sphingomyelin levels and inhibits cell proliferation of HepG2 and Caco-2 cells

Fig. 3

Changes of acid SMase mRNA (upper panel) and Western blot for acid SMase protein (lower panel) in HepG2 cells. The cells were incubated with mevalonate at different concentrations for 24 h. The total RNA was extracted and cDNA synthesized. QPCR was performed on Bio-Rad iCycler system. The results were normalized with the levels of the housekeeping gene (GAPDH) and expressed as ratio of the control. The data are expressed as means ± SD obtained from duplicate culture wells in three separate experiments. The Western blot (lower panel) for acid SMase was performed in HepG2 cells incubated with mevalonate at concentrations indicated for 24 h. No statistical significance was identified

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Lipids in Health and Disease

ISSN: 1476-511X