Fig. 2

Effects of resveratrol on autophagy-related protein expressions in mice livers and AML12 cells. Mice were fed a normal diet (Control) or the MCD diet (MCD) with or without 100 mg/kg resveratrol (MCD + Res 100), 250 mg/kg resveratrol (MCD + Res 250) for 4 weeks. AML12 cells were incubated with resveratrol (25 μmol/L, 50 μmol/L, or 100 μmol/L) in the presence or absence of MCD medium for 24 h. LC3 and P62 levels were evaluated by immunoblotting both in mice livers (a,b) and in AML12 cells (c,d). Levels of LC3 and P62 were determined and the protein bands were quantified by densitometry and normalized to the amount of GAPDH in hepatic tissue and AML12 cells. The data were represented as mean ± SD. *P < 0.05, **P < 0.01 v.s. Control; ^# P < 0.05, ^## P < 0.01 v.s. MCD