Combined incubation of Cadmium, docosahexaenoic and eicosapentaenoic acid results in increased uptake of cadmium and elevated docosapentaenoic acid content in Hepatocytes in vitro

Table 1 Fatty acid content in Hep G2 cells

μg FA per Mio cells	BSA-control no FA	BSA-FA (24 h)	BSA-control no FA Cd5 (24 h)	BSA-FA (24 h) Cd5 (24 h)
C20:5n-3	0.09 ± 0^a	0.37 ± 0.09^b	0.14 ± 0.05^a	0.59 ± 0.14^b
C22:5 n-3	0.10 ± 0.01^a	0.16 ± 0.03^ab	0.17 ± 0.07^ab	0.31 ± 0.06^b
C22:6n-3	0.40 ± 0.06^a	0.84 ± 0.08^b	0.57 ± 0.22^a	1.23 ± 0.19^b
SFA	5.61 ± 0.98	5.47 ± 0.66	7.24 ± 3.20	8.38 ± 2.19
MUFA	8.19 + 0.98^a	6.02 ± 0.33^b	8.65 ± 2.88^ab	7.48 ± 1.30^b
PUFA	2,00 ± 0.29^a	2.60 ± 0.28^b	2.84 + 1.08^ab	4.01 ± 0.44^b
n-3	0.59 ± 0.07^a	1.38 ± 0.10^b	0.88 ± 0.34^a	2.13 ± 0.23^b
n-6	1.36 ± 0.26^ab	1,20 ± 0,19^b	1.94 ± 0.76^a	1.81 ± 0.30^ab
n-6/n-3	2.28 ± 0.18^a	0.87 ± 0.08^b	2.21 ± 0.02^a	0.85 ± 0.08^b

FA content (% of total identified) in Hep G2 cells incubated with or without FA (24 h) and subsequently with or without 5 μM Cd²⁺ for 24 h. ‘No FA’ means cells were only incubated with BSA as control. Data are presented as means ± SD, n = 3. Different letters denote significant differences between treatments (two factorial ANOVA, (FA, Cd²⁺), p < 0.05)
Abbreviations: PC L a-Phosphatidylcholine, CL Cardiolipin, PA L-a-Phosphatidic acid, and EA Phosphatidylethanolamine

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