Fig. 3From: Cigarette smoke exposure impairs reverse cholesterol transport which can be minimized by treatment of hydrogen-saturated salineMeasurements of HDL functionalities. Levels of MDA, thiobarbituric acid reactive substances (TBARS), were measured to assess the extent of LDL oxidation (Panel a). Plasma SOD levels was determined (Panel b). [3H]-cholesterol-laden macrophages were co-cultured with HDL from each group to determine the HDL functionality to promote cholesterol efflux (Panel c). [3H] cholesterol efflux % was calculated by dividing the supernatant-derived radioactivity by the sum of the supernatant-derived radioactivity and the intracellular macrophage radioactivity. Values were indicated as means ± S.D. (n = 6). Significantly different from respective control: *p < 0.05Back to article page