Fig. 2

CETP expression does not impact on insulin secretion. Freshly isolated islets from female NTg (white columns) or CETP (gray columns) mice with 7 month of age were incubated in Krebs bicarbonate buffer containing 5.6 mmol/l glucose for 30 min at 37 °C (5 islets/well, four replicates in each condition). Then, the buffer was replaced by fresh buffer containing 2.8 mM glucose, 2.8 mM glucose plus KCl (40 mM), 2.8 mM glucose plus leucine (10 mM), 11 mM glucose or 16.7 mM glucose. After 1 h incubation, buffer was collected and insulin was measured by radioimmunoassay. Insulin release was normalized by islet number. Values are expressed as mean ± S.E. n = 4–6 mice in 3 independent experiments. Student’s t test: non-significant