Table 1 Genes and primers sets for real-time PCR
From: Effect of n-3 fatty acids on the expression of inflammatory genes in THP-1 macrophages
5’ Forward primer 3’ | |
|---|---|
Reverse primer | |
NFKB pathway : | |
AKT1 | CTCCTGAGGAGCGGGAGGAGTGG |
GTCCACTCCTCCCGCTCCTCAGGA | |
MAPK | TATGGCTCTGTGTGTGCTGCTTTTG |
CAAAAGCAGCACACACAGAGCCAT | |
NFKB | ACAACTATGAGGTCTCTGGGGGTA |
GTACCCCCAGAGACCTCATAGTTGT | |
Cytokines production : | |
TNFA | CCATGTTGTAGCAAACCCTCAAGCT |
AGCTTGAGGGTTTGCTACAACATGG | |
IL1Β | CAGATGAAGTGCTCCTTCCAGGACC |
GGTCCTGGAAGGAGCACTTCATCTG | |
MCP1 | CGCTCAGCCAGATGCAATCAATGCC |
GGCATTGATTGCATCTGGCTGAGCG | |
Fatty acids metabolism : | |
ALOX5 | GGCATTGATTGCATCTGGCTGAGCG |
CGCTCAGCCAGATGCAATCAATGCC | |
PTGS2 | CTGGGCCATGGGGTGGACTTAAATC |
GATTTAAGTCCACCCCATGGCCCAG | |
Oxydation system : | |
MGST1 | TTGACAAGAAAGGTTTTTGCCAATC |
GATTGGCAAAAACCTTTCTTGTCAA | |
NOS2 | CCATGGAACATCCCAAATACGAGT |
CACTCGTATTTGGGATGTTCCATG | |
HO-1 | AAGATTGCCCAGAAAGCCCTGGAC |
AACTGTCGCCACCAGAAAGCTGAG | |
Housekeeping genes : | |
RPL37A | |
GGTGCCTGGACGTACAATACCACTT | |
AAGTGGTATTGTACGTCCAGGCACC | |
18S | CCATTGGAGGGCAAGTCTGGTGCC |
TGGCACCAGACTTGCCCTCCAATGG |