Effects of n-3 polyunsaturated fatty acids high fat diet intervention on the synthesis of hepatic high-density lipoprotein cholesterol in obesity-insulin resistance rats

Table 3 Primers for analysis of transcription level of gene expression

Gene	Accession no.	Primer	Tm, °C	Product length,bp
ABCA1	NM_178095.2	F 5′-AGAGCTAGGTCTCCCTT-3′	56	151
ABCA1	NM_178095.2	R 5′-CACTGCCCCTGTAATGG-3′	56	151
apoA-1	NM_012738	F 5′-GAACAAGGACCTGGAGAATG-3′	58	315
apoA-1	NM_012738	R 5′-CTGGCCTTGGTATGATACTC-3′	58	315
actin	NM_031144.2	F 5′-CCCATCTATGAGGGTTACGC-3′	50	150
actin	NM_031144.2	R 5′-TTTAATGTCACGCACGATTTC-3′	50	150

ISSN: 1476-511X