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Table 3 Primers for analysis of transcription level of gene expression

From: Effects of n-3 polyunsaturated fatty acids high fat diet intervention on the synthesis of hepatic high-density lipoprotein cholesterol in obesity-insulin resistance rats

Gene Accession no. Primer Tm, °C Product length,bp
ABCA1 NM_178095.2  F 5′-AGAGCTAGGTCTCCCTT-3′ 56 151
R 5′-CACTGCCCCTGTAATGG-3′
apoA-1 NM_012738 F 5′-GAACAAGGACCTGGAGAATG-3′ 58 315
R 5′-CTGGCCTTGGTATGATACTC-3′
actin NM_031144.2 F 5′-CCCATCTATGAGGGTTACGC-3′ 50 150
R 5′-TTTAATGTCACGCACGATTTC-3′