Fig. 2

Identification of G418-resistant clones and hfat-1 transgenic pigs. Analyse of the hfat-1 gene and neomycin gene integration into the pig genome in the G418-resistant clones were performed using a PCR assay with the primer pairs of hfat-1 identi (458 bp) (a) and neo identi (790 bp) (b). a 1–5, five G418-resistant clones, on which SCNT was individually performed in the following procedure. 6, hfat-1 plasmid as a positive control. 7–8, negative controls. b 1, hfat-1 plasmid as a positive control. 2–3, negative controls. 4–8, five G418-resistant clones. The hfat-1 gene integration into the pig genome and transcription in the transgenic pigs were analyzed using PCR (c) and RT-PCR (d), respectively, with the hfat-1 identi primer pair (458 bp). c 1–5, five hfat-1 transgenic pigs. 6, hfat-1 plasmid as a positive control. 7–8, two wild-type pigs. d 1–5, five hfat-1 transgenic pigs. 6–7, two wild-type pigs