Table 1 Primer sequences of genes selected for analysis by real-time RT-PCR
Genes | GenBank accession | Primer sequence (5′ ~ 3′) | Product length (bp) | Tm (°C) |
|---|---|---|---|---|
HSPA1L | NM_001123128.1 | F:CGCTTTGACCTGACTGGAAT | 120 | 60 |
R:CTTGCCTGTGCTCTTGTCC | ||||
CD8A | NM_001001907.1 | F:GCTGGACACCCGTTACATCT | 100 | 60 |
R:CGAGCAGAAATAGTAGCCTTGG | ||||
CD40 | NM_214194.1 | F:GGTTCGTCTGCCTCTGAAGT | 104 | 60 |
R:GGCTGTTTGTTGGGTATTGG | ||||
PSTPIP1 | NM_001244186.1 | F:CTCCTTTGACTCCCTGAAGC | 114 | 60 |
R:TTCTGCCTCTCTCGGAACTC | ||||
SLA-DQA1 | NM_001114062.2 | F:TGGACCTGGAGAAGAAGGAG | 132 | 60 |
R:TGGAGCGTTTAGTCACGATG | ||||
STAT2 | NM_213889.1 | F:TCCCAAATCACAAGGTTTCC | 109 | 60 |
R:CAGATAGCCGAAGTCCCAAA | ||||
GK | NM_001143708.1 | F:GCAGGTAGATGGAGGGATGA | 107 | 60 |
R:CCAGGGCAGTTGTTTCAGG | ||||
BMP7 | NM_001105290.1 | F:TCCAGGGCAAGCACAACT | 172 | 60 |
R:TCGGTGAGGAAGTGGCTATC | ||||
PIK3R5 | NM_213851.1 | F:CTGTCATTCCCTCCTTCCAA | 117 | 60 |
R:GCCACCCTCCTCTTACTCTG | ||||
SLA-DRB1 | NM_001113695.1 | F:TCTGCTCTTTGTTGCTGTGG | 120 | 60 |
R:GGATGCTTGCTTGGAGTGTC | ||||
THY1 | XM_005667396.1 | F:GGCATCGCTCTCTTGCTAAC | 125 | 60 |
R:GGCAGGTTGGTGGTATTCTC | ||||
TGFB1 | NM_214015.1 | F:AAGCGGCAACCAAATCTATG | 113 | 60 |
R:CCCGAGAGAGCAATACAGGT | ||||
SLA-1 | NM_001097431.1 | F:GTCAAGGAAACCGCACAGAT | 113 | 60 |
R:CCCAAGTAGCAGCCAAACAT | ||||
CD74 | NM_213774.1 | F:ATGGACGGTGTGAACTGGA | 100 | 60 |
R:GAACCTCAAAGGGTGTCTCCT | ||||
SOD2 | XM_005659113.1 | F:CCTTCACTTTGCCTCTTGGT | 127 | 60 |
R:CACCGTTAGGGCTCAGATTT | ||||
ACTA2 | XM_005671254.1 | F:GTCCACCTTCCAGCAAATGT | 105 | 60 |
R:AGACAGCGAGCAGGGTAAGT | ||||
SULT1E1 | NM_213992.1 | F:TGAAGTCTCATCTGCCACCT | 101 | 60 |
R:AGAAACGACCACATCCTTGG | ||||
β-actin | DQ845171.1 | F:GGCGCCCAGCACGAT | 66 | 60 |
R:CCGATCCACACGGAGTACTTG |