Table 1 Advantages and disadvantages of clinical DNL techniques
From: Clinical assessment of hepatic de novo lipogenesis in non-alcoholic fatty liver disease
DNL Technique | Advantages | Disadvantages |
|---|---|---|
Stable label isotope | Fluxed based assay Selection of stable isotopes Moderate sensitivity Reproducible results | Cost of stable label substrates Enrichment period (up to 7Â days prior to experiment) Requires specialized equipment for analysis |
Fatty acid profiling | Relatively inexpensive Simple to execute High sensitivity High throughput | Requires specialized equipment for analysis |
Indirect calorimetry | Non-invasive (no blood sample required) | Not hepatic specific |