Fig. 1

Topical rHDL promotes wound closure and wound angiogenesis in aged mice. Two full thickness wounds were created on the back flanks of 24-month-old C57Bl/6 J mice (n = 9/group). Mice received daily topical applications of rHDL (50 μg/wound) or PBS (vehicle). a Wound area was calculated from the average of three daily diameter measurements of the x, y and z-axes. Wound closure is expressed as a percentage of initial wound area at Day 0; b Blood flow perfusion was determined by laser Doppler imaging. Upper panels are representative Doppler images that depict high (red) to low (blue) wound blood flow taken at Days 2 and 10. Blood perfusion was determined based on rHDL:PBS wound ratio, with values above 100 % indicating increased blood perfusion with rHDL treatment relative to PBS control. Data is represented as mean ± SEM. Statistical analysis was performed by unpaired two-tailed t-test. *p < 0.05, **p < 0.01 vs. PBS treated wounds