Fig. 7

The effect of rHDL on angiogenic markers in ischemic hindlimbs. Femoral artery ligation was performed on 24-month-old C57B1/6 J mice. Mice received i.v. injections of PBS or rHDL (40 mg/kg) on alternate days following ligation until sacrifice 21 days later. On Day 21 post-surgery hindlimb tissue was collected and RNA isolated for qRT-PCR on non-ischemic (NON) and ischemic (ISC) hindlimbs to assess gene expression of (a) Siah1; (b) Siah2; (c) PHD2; (d) PHD3; (e) HIF-1α; and (f) VEGF, normalized to β2-microglobulin (B2M). g VEGF protein was determined on homogenized hindlimbs using ELISA. Data is represented as mean ± SEM. Statistical analysis was performed by one-way ANOVA and Bonferroni’s comparison test post hoc. *p < 0.05 vs. PBS controls