Fig. 4

The promotion of BPA on NPC1L1 expression and cholesterol absorption may be mediated through SREBP-2. a Expression analysis of SREBP-2 in Caco-2 cells after 24 h of stimulation with increasing concentrations of BPA. Data are presented as means ± SEM of three independent experiments. *P < 0.05, ***P < 0.001 compared to untreated cells. b Up-regulation of NPC1L1 expression in Caco-2 cells after 24 h incubation with BPA, by transfecting short interfering RNA (siRNA) for SREBP-2 compared with scramble control transfections. Data are presented as means ± SEM of three independent experiments. *P < 0.05 compared to untreated cells. c After the inhibition of SREBP-2 expression using siRNA, Caco-2 cells were incubated with BPA for 24 h, then incubated with radioactive micellar cholesterol for additional 2 h. The absorption of cholesterol in the absence of BPA was normalized to 100%. Results are mean ± SEM from triplicate determinations in three separate experiments. **P < 0.01 compared to untreated cell