Fig. 3
From: Pigment epithelium-derived factor alleviates endothelial injury by inhibiting Wnt/β-catenin pathway
Wnt/β-catenin pathway mediates ox-LDL-induced cytotoxicity in HUVECs. HUVECs were pretreated with 20 nM DKK-1 or 20 mM LiCl for 24 h in the presence or absence of incubation with 100 mg/L ox-LDL for 24 h. Cell viability (a) and LDH activity in the medium (b) were assessed by an MTT assay and an assay kit, respectively. c The apoptotic cells stained with Annexin V-FITC and PI were detected by flow cytometry. All data are expressed as the mean ± SD of at least 6 independent experiments. *P < 0.05, **P < 0.01 versus the control group; # P < 0.05, ## P < 0.01 versus the ox-LDL group. LiCl, an exogenous activator of Wnt/β-catenin pathway; DKK-1, an inhibitor of Wnt/β-catenin pathway