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Fig. 7 | Lipids in Health and Disease

Fig. 7

From: HDL-associated ApoM is anti-apoptotic by delivering sphingosine 1-phosphate to S1P1 & S1P3 receptors on vascular endothelium

Fig. 7

S1P1 or S1P3 antagonists block AKT and ERK phosphorylation. a Cells were pre-incubated with ± W146 1 μM or CAY10444 10 μM for 30 min and then treated with free S1P 1 μM, ApoM 1 μM, ApoM-bound S1P 1 μM or albumin-bound S1P 1 μM for 10 min. Then, cells were lysed and analyzed by western blot for pAKT and pERK. Total AKT, ERK and GAPDH were analyzed as controls. b Quantification of relevant pairs of A. Student t-test of n = 3 independent experiments. Error bars correspond to SD. *p < 0.05

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Lipids in Health and Disease

ISSN: 1476-511X