Table 6 Fatty acid composition of testicle phospholipids from control mice and high-fat diet (HFD) fed mice receiving hydroxytyrosol (HT) supplementation

C16:0

30.6 ± 3.0^c

29.2 ± 2.7^c

41.6 ± 3.9^a,b,d

29.5 ± 2.9^c

C18:0

7.51 ± 1.2

6.99 ± 0.8

6.21 ± 1.1

7.44 ± 0.9

C18:1n-9

22.6 ± 2.0

23.9 ± 2.4

21.8 ± 1.9

24.5 ± 2.6

C18:2n-6 (LA)

11.6 ± 0.6

12.8 ± 0.9

10.6 ± 0.6

11.2 ± 0.5

C18:3n-3 (ALA)

2.06 ± 0.2^c

2.21 ± 0.2^c

0.56 ± 0.05^a,b,d

1.98 ± 0.1^c

C20:4n-6 (AA)

8.54 ± 0.7

9.05 ± 0.9

8.40 ± 0.8

8.38 ± 0.7

C20:5n-3 (EPA)

1.19 ± 0.2^c

1.36 ± 0.1^c

0.46 ± 0.05^a,b,d

1.25 ± 0.2^c

C22:5n-6 (DPAn-6)

0.38 ± 0.06^c

0.45 ± 0.07^c

0.12 ± 0.03^a,b,d

0.41 ± 0.06^c

C22:5n-3 (DPAn-3)

0.44 ± 0.05^c

0.49 ± 0.06^c

0.15 ± 0.02^a,b,d

0.43 ± 0.07^c

C22:6n-3 (DHA)

10.2 ± 1.1^c

10.6 ± 1.4^c

4.58 ± 0.8^a,b,d

9.36 ± 1.2^c

Total SFAs

38.9 ± 3.3^c

36.8 ± 3.1^c

48.2 ± 4.1^a,b,d

38.4 ± 3.4^c

Total MUFAs

25.7 ± 2.5

26.6 ± 2.2

24.9 ± 2.1

27.6 ± 2.4

Total PUFAs

35.4 ± 3.1^c

36.6 ± 3.4^c

26.9 ± 2.2^a,b,d

34.0 ± 3.0^c

Total LCPUFAs

21.1 ± 1.9^c

22.5 ± 2.2^c

13.9 ± 1.0^a,b,d

20.5 ± 2.0^c

Total n-6 LCPUFAs

9.12 ± 0.8

9.70 ± 0.9

8.70 ± 0.7

9.00 ± 0.8

Total n-3 LCPUFAs

11.9 ± 1.1^c

12.8 ± 1.4^c

5.20 ± 0.5^a,b,d

11.5 ± 0.9^c

n-6/n-3 LCPUFA ratio

0.77 ± 0.05^c

0.76 ± 0.04^c

1.67 ± 0.3^a,b,d

0.78 ± 0.04^c