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Table 6 Fatty acid composition of testicle phospholipids from control mice and high-fat diet (HFD) fed mice receiving hydroxytyrosol (HT) supplementation

From: Hydroxytyrosol prevents reduction in liver activity of Δ-5 and Δ-6 desaturases, oxidative stress, and depletion in long chain polyunsaturated fatty acid content in different tissues of high-fat diet fed mice

  Fatty acid composition (g/100 g FAME)
  Groups
  Control diet (CD) High fat diet (HFD)
  Saline HT Saline HT
Fatty acid (a) (b) (c) (d)
C16:0 30.6 ± 3.0c 29.2 ± 2.7c 41.6 ± 3.9a,b,d 29.5 ± 2.9c
C18:0 7.51 ± 1.2 6.99 ± 0.8 6.21 ± 1.1 7.44 ± 0.9
C18:1n-9 22.6 ± 2.0 23.9 ± 2.4 21.8 ± 1.9 24.5 ± 2.6
C18:2n-6 (LA) 11.6 ± 0.6 12.8 ± 0.9 10.6 ± 0.6 11.2 ± 0.5
C18:3n-3 (ALA) 2.06 ± 0.2c 2.21 ± 0.2c 0.56 ± 0.05a,b,d 1.98 ± 0.1c
C20:4n-6 (AA) 8.54 ± 0.7 9.05 ± 0.9 8.40 ± 0.8 8.38 ± 0.7
C20:5n-3 (EPA) 1.19 ± 0.2c 1.36 ± 0.1c 0.46 ± 0.05a,b,d 1.25 ± 0.2c
C22:5n-6 (DPAn-6) 0.38 ± 0.06c 0.45 ± 0.07c 0.12 ± 0.03a,b,d 0.41 ± 0.06c
C22:5n-3 (DPAn-3) 0.44 ± 0.05c 0.49 ± 0.06c 0.15 ± 0.02a,b,d 0.43 ± 0.07c
C22:6n-3 (DHA) 10.2 ± 1.1c 10.6 ± 1.4c 4.58 ± 0.8a,b,d 9.36 ± 1.2c
Total SFAs 38.9 ± 3.3c 36.8 ± 3.1c 48.2 ± 4.1a,b,d 38.4 ± 3.4c
Total MUFAs 25.7 ± 2.5 26.6 ± 2.2 24.9 ± 2.1 27.6 ± 2.4
Total PUFAs 35.4 ± 3.1c 36.6 ± 3.4c 26.9 ± 2.2a,b,d 34.0 ± 3.0c
Total LCPUFAs 21.1 ± 1.9c 22.5 ± 2.2c 13.9 ± 1.0a,b,d 20.5 ± 2.0c
Total n-6 LCPUFAs 9.12 ± 0.8 9.70 ± 0.9 8.70 ± 0.7 9.00 ± 0.8
Total n-3 LCPUFAs 11.9 ± 1.1c 12.8 ± 1.4c 5.20 ± 0.5a,b,d 11.5 ± 0.9c
n-6/n-3 LCPUFA ratio 0.77 ± 0.05c 0.76 ± 0.04c 1.67 ± 0.3a,b,d 0.78 ± 0.04c
  1. Values are expressed as g fatty acid per 100 g FAME and represent the mean ± SEM for n = 12–14 mice per experimental group. Significant differences between the groups are indicated by the letter identifying each group (one-way ANOVA and Newman-Keuls´ post-test; p < 0.05). The identification of saturated and unsaturated fatty acids and their relationships are shown in Table 2