Table 2 Detection of Cyclin D1、p53、P16、P27、P21 and Rb mRNA expression by Quantitative Real Time PCR
From: E167K polymorphism of TM6SF2 gene affects cell cycle of hepatocellular carcinoma cell HEPA 1-6
groups | Quantitative Real Time PCR | |||||
|---|---|---|---|---|---|---|
Cyclin D1 | p53 | Rb | P27 | P21 | P16 | |
variant type group | 2.03 ± 0.01 | 1.88 ± 0.05 | 1.29 ± 0.06 | 0.56 ± 0.02 | 0.78 ± 0.06 | 1.00 ± 0.04 |
wild type group | 1.00 ± 0.00 | 1.48 ± 0.09 | 1.18 ± 0.01 | 0.82 ± 0.05 | 0.77 ± 0.02 | 1.01 ± 0.06 |
the control group | 1.04 ± 0.06 | 1.37 ± 0.03 | 1.15 ± 0.03 | 0.85 ± 0.05 | 0.78 ± 0.04 | 1.00 ± 0.06 |
t1 | 178.532 | 6.729 | 3.132 | 8.363 | 0.158 | 0.240 |
P1 | <0.001 | 0.0016 | 0.041 | <0.001 | >0.05 | >0.05 |
t2 | 28.190 | 15.152 | 3.615 | 9.328 | 0 | 0 |
P2 | <0.001 | <0.001 | 0.014 | <0.001 | >0.05 | >0.05 |
t3 | 1.156 | 2.008 | 1.643 | 0.735 | 0.387 | 0.204 |
P3 | >0.05 | >0.05 | >0.05 | >0.05 | >0.05 | >0.05 |