Fig. 2
From: Overexpression of apolipoprotein A-I alleviates endoplasmic reticulum stress in hepatocytes
ApoA-I overexpression downregulates lipid accumulation and alters SREBP-1, FAS and ACC1 protein expression in HepG2 cells. HepG2 cells were transfected with pcDNA3.0 alone or with pcDNA3.0/apoA-I plasmid using the FuGENE HD transfection reagent. Twenty-four hours after transfection, the transfected cells were incubated for 24 h in DMEM in the presence or absence of 2 μg/mL TM, and levels of cellular triglycerides (a) as well as free cholesterol (b) were measured. In addition, total cellular proteins were isolated and analysed for p-SREBP1, m-SREBP1 (c), FAS (d) and p-ACC1/ACC1 (e) by Western blotting. The results are representative of three independent experiments and are presented as the mean ± SEM. *P < 0.05 versus control. **P < 0.01 versus control. ***P < 0.001 versus control