Fig. 6
From: The purification of reduced β2-glycoprotein I showed its native activity in vitro
Reduced β2GPI protected HUVECs from oxidative stress-induced cell death. Reduced β2GPI increased the HUVECs cell viability (a) and cell number (b) upon the H2O2 treatment. (c) The image of the cell apoptosis by FACS. (d) Reduced β2GPI protected HUVECs from apoptosis under H2O2 treatment. HUVECs incubated with β2GPI (1 μM) were pretreated with TRX-1 (1.75 μM) + DTT (35 μM) + GSH (80 mM) for 30 min at 37 °C and then incubated with 4 mM H2O2 for 40 min at 37 °C. No difference was observed (n = 5) between H2O2-only-treated cells and β2GPI only or TRX-1 (1.75 μM) + DTT (35 μM) + GSH (80 mM). For all panels, *P < 0.05 and **P < 0.001