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Table 1 Composition of washing solutions

From: Lipase and biosurfactant from Ochrobactrum intermedium strain MZV101 isolated by washing powder for detergent application

  Volume (ml)
Solutions B B + L B + S B + D + L B + D + S B + D + L + S
0.1 M Tris buffer pH = 8.5 40 40 40 40 40 40
Lipase 10 10 10
Biosurfactant 10 10 10
1% Detergent solution 40 40 40
Distilled water 60 50 50 10 10
  1. B = 0.1 M Tris buffer pH = 8.5
  2. L = lipase (1000 U)
  3. S = biosurfactant (1000 U)
  4. D = 1% Detergent solution
  5. The stained fabrics were incubated in 250 ml flasks containing 100 ml washing solutions as mentioned below. All tests were repeated at least in triplicate independent experiments