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Table 1 Composition of washing solutions

From: Lipase and biosurfactant from Ochrobactrum intermedium strain MZV101 isolated by washing powder for detergent application

 

Volume (ml)

Solutions

B

B + L

B + S

B + D + L

B + D + S

B + D + L + S

0.1 M Tris buffer pH = 8.5

40

40

40

40

40

40

Lipase

–

10

–

10

–

10

Biosurfactant

–

–

10

–

10

10

1% Detergent solution

–

–

–

40

40

40

Distilled water

60

50

50

10

10

–

  1. B = 0.1 M Tris buffer pH = 8.5
  2. L = lipase (1000 U)
  3. S = biosurfactant (1000 U)
  4. D = 1% Detergent solution
  5. The stained fabrics were incubated in 250 ml flasks containing 100 ml washing solutions as mentioned below. All tests were repeated at least in triplicate independent experiments