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Fig. 5 | Lipids in Health and Disease

Fig. 5

From: Quantification of sterol-specific response in human macrophages using automated imaged-based analysis

Fig. 5

Results of MTT assay for all conditions. The MTT assay of the number of viable cells in each condition was carried out according to manufacturer instructions. Results were quantified with a multiplate reader and calibrated against standards generated from the same cell stock. -ApoB (pre-cultured with rabbit anti-apolipoprotein B antibodies), Chol (cholesterol and 1-palmitoyl-lyso-phosphatidylcholine (LPC) in PBS), CHS (cholesteryl hemisuccinate and LPC in PBS), Erg (ergosterol and LPC in PBS), LDL (low-density lipoprotein in PBS), LPC (LPC in PBS), LPS (lipopolysaccharide in PBS), PBS (phosphate-buffered saline vehicle), Ser (medium contained 10% fetal bovine serum)

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