Fig. 4

NFKB participates in Slc2a4 gene downregulation by palmitate in L6 cells. L6 myotubes were treated with 0.5% ethanol (vehicle) for 12 h (control sample, C) or 0.75 mM palmitate for 2, 6 or 12 h. a and b Relative nuclear protein content of complexes a and b (Slc2a4-NFKB); c EMSA competition assay (left panel) and representative image of EMSA assay showing complexes a and b (right panel); d Relative NFKB p65 nuclear protein content normalized by total protein analysis with Coomassie Blue-stained gel. Values are means ± SEM of 10 (a), 9 to 10 (b), and 5 to 10 (d) samples; *P < 0.05 and **P < 0.01 versus C; #P < 0.05 versus 2 h, §P < 0.05 versus 6 h; &P < 0.05 versus 12 h, one-way ANOVA, Student–Newman–Keuls as post hoc test