Fig. 3

Effects of autophagy inhibition at different stages of differentiation on oleic acid (OA)-induced adipogenesis in hADSCs. a Adipogenic differentiation in hADSCs was induced with 80 μM OA, and the cells were treated with 3MA (10 mM) for different periods of time. b Western blot analysis of LC3II/I in hADSCs treated with 3MA for 24 h. c Oil red O staining at the end of differentiation (day 10). d Quantification of intracellular lipids at day 10. e Western blot analysis of PPARγ and C/EBPα at day 10. Data are shown as the mean ± SD (n = 3). ^**p < 0.01 vs. control