Fig. 1

Analysis of LOX-1 in bone marrow cells (BMCs) and bone marrow macrophages (BMMs). (A, upper panel, B, C) BMCs were differentiated into BMMs with or without Pam3CSK4 (TLR2 ligand, 100 ng/ml) or Lipid A (TLR4 ligand, 100 ng/ml) in the presence of macrophage colony-stimulating factor (M-CSF, 20 ng/ml) for 3 days. (A, lower panel) BMMs were cultured with or without Pam3CSK4 (100 ng/ml) or Lipid A (100 ng/ml) in the presence of M-CSF and receptor activator of nuclear factor kappa-B ligand (RANKL, 50 ng/ml) for 3 days. a Polymerase chain reaction (PCR) products of LOX-1, a receptor of oxidative low density lipoprotein (oxLDL), mRNA in BMCs (upper panel) and BMMs (lower panel) were amplified using RT-PCR methods. Following stimulation with Pam3CSK4 or Lipid A, Lox-1 expression was upregulated in BMCs (upper panel) but not in BMMs (lower panel). b Lox-1 mRNA expression peaked on day 1 or 3 as determined by real-time PCR (left panel) and protein expression on day 3 (right panel). c BMCs stimulated with TLR ligands on day 3 were stained with anti-Lox-1 antibody (1:100 dilution) then incubated with Alexa fluor 488-conjugated streptavidin (2 μg/ml) in goat anti-rabbit IgG secondary antibody (1:1500 dilution). Lox-1 expression was upregulated. Data are expressed as mean ± SEM (n = 3). *P < 0.05 compared with control