Table 2 Sequences of primers and probes for real-time RT-PCR
Gene | Primer / Probe | Sequence (5’ to 3’) |
|---|---|---|
Mouse ApoM | Forward | GCTTTCTCCTCTACAATCGGTCAC |
Reverse | CGGGCAGGCCTCTTGATT | |
Probe | FAM- ACCTCTTGCTTGGACTTCAAAGCCTTCTTA-TAMRA | |
Mouse ApoAI | Forward | CAGTTTGAATCCTCCTCCTTGG |
Reverse | GGTTATCCCAGAAGTCCCGAG | |
Probe | FAM-CAACAGCTGAACCTGAATCTCCTGGAA-TAMRA | |
Mouse GAPDH | Forward | TCTTGTGCAGTGCCAGCCT |
Reverse | TGAGGTCAATGAAGGGGTCG | |
Probe | FAM-AGGTCGGTGTGAACGGATTTGGC-TAMRA | |
Human ApoM | Forward | CTGACAACTCTGGGCGTGGAT |
Reverse | TGTCCACAGGGTCAAAAGTTGC | |
Probe | FAM-AGTTCCCAGAGGTCCACTTGGGCCA-BHQ1 | |
Human GAPDH | Forward | CAGGGCTGCTTTTAACTCTGGT |
Reverse | CATGGGTGGAATCATATTGGAAC | |
Probe | CY5-TGGATATTGTTGCCATCAATGACCCCT-BHQ2 |