Fig. 3
From: Exercise intervention alters HDL subclass distribution and function in obese women
Changes in paraoxonase activity and protein expression in response to the intervention. Paraxonase activity of diluted sera was measured at A405 over a 20 min time interval using the paraoxon-ethyl substrate. One unit of activity is defined as 1 nmol of substrate disintegrated per minute (a). Participant sera (b and d) and isolated HDL (c and e) were run on reducing 12.5% SDS-PAGE gels and transferred to nitrocellulose membrane. Ponceau S staining was used to confirm equal loading. Blots were probed with mouse anti-PON-1 antibody. Results are representative of randomized experiments (b) and (c). Results are presented as percentage changes in densitometry relative to a baseline in sera (d) and HDL (e). Results are means ± SEM.* p < 0.05 significance for interaction. PON, Paraoxonase