Fig. 4

P62 and LC3 expression in cardiac tissues of the four groups after 16 weeks under different treatments. a Representative immunohistochemistry for p62 and LC3 in cardiac tissues. Scale bar = 100 μm. Arrows indicate positively stained cells. b Relative mRNA expression of p62 and LC3 in cardiac tissue of each group after 16 weeks under different treatments. c Immunoblotting for p62 0061nd LC3 in cardiac tissues. d Bar graph showing quantification of p62 and LC3 protein expression. Data are given as the means ± SEM; n = 5–6 in each group. * P < 0.05 vs. ApoE^−/− HD