Table 1 The sequences of forward and backward primers, restriction enzymes for genotyping of the LIPG SNPs
From: LIPG SNPs, their haplotypes and gene-environment interactions on serum lipid levels
SNP | Primer sequence | PCR product | Restriction enzyme | Restriction fragment | Allele |
|---|---|---|---|---|---|
rs3813082 | 5'-GACACCCAGATCCTCCTCTC-3' 3'-AGGAGGACAAAGGGGATGAC-5' | 210 bp | HhaI | 210 117 + 93 | G T |
rs2000813 | 5'-CATGAGCTGAGATTGTTGTCAGTGC-3' 3'-CAGTCAACCACAACTACATTGGCGTCTTTCTCTCAT-5' | 254 bp | NdeI | 254 217 + 37 | C T |
rs2097055 | 5'-TCAGGATTCTCGAGCAGTCC-3' 3'-CTTAGGGGAGGCCAAAAGGA-5' | 421 bp | BccI | 421 283 + 138 | C T |
rs3819166 | 5'-CCGGACGATGCAGATTTTGT-3' 3'-CATTGCACTCTAACCTGGGC-5' | 473 bp | SspI | 473 307 + 166 | G A |