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Table 1 The sequences of forward and backward primers, restriction enzymes for genotyping of the LIPG SNPs

From: LIPG SNPs, their haplotypes and gene-environment interactions on serum lipid levels

SNP Primer sequence PCR product Restriction enzyme Restriction fragment Allele
rs3813082 5'-GACACCCAGATCCTCCTCTC-3'
3'-AGGAGGACAAAGGGGATGAC-5'
210 bp HhaI 210
117 + 93
G
T
rs2000813 5'-CATGAGCTGAGATTGTTGTCAGTGC-3'
3'-CAGTCAACCACAACTACATTGGCGTCTTTCTCTCAT-5'
254 bp NdeI 254
217 + 37
C
T
rs2097055 5'-TCAGGATTCTCGAGCAGTCC-3'
3'-CTTAGGGGAGGCCAAAAGGA-5'
421 bp BccI 421
283 + 138
C
T
rs3819166 5'-CCGGACGATGCAGATTTTGT-3'
3'-CATTGCACTCTAACCTGGGC-5'
473 bp SspI 473
307 + 166
G
A