Fig. 2

ABC transporter gene expression in liver, kidney and white adipose tissue of 12-month-old Abcc6^−/− mice. Normalized relative gene expression level of various ABC transporters in the (a) liver (b) kidney and (c) WAT of 12-month-old ± 4 weeks male and female WT (liver: n = 37; kidney: n = 34; WAT: n = 33, respectively) and Abcc6^−/− mice (liver: n = 39; kidney: n = 42; WAT: n = 38, respectively) by qRT-PCR. Relative mRNA expression of ABC transporter members belonging to a, b, c, d and g subfamily. Data are presented as fold change of mean ± SEM relative to samples of age-matched WT. Statistically significant differences were analyzed using unpaired, two-tailed Student’s t-test and are indicated in the following manner: * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001