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Fig. 3 | Lipids in Health and Disease

Fig. 3

From: Trans-vaccenic acid inhibits proliferation and induces apoptosis of human nasopharyngeal carcinoma cells via a mitochondrial-mediated apoptosis pathway

Fig. 3

TVA induces apoptosis in NPC cells through Akt and Bad inactivation. a. The expression of Akt, p-Akt, Bad, p-Bad and Mcl-1 in 5-8F cells was analyzed by western blot assay after the cells were treated with TVA. b. The expression of Akt, p-Akt, Bad, p-Bad and Mcl-1 in CNE-2 cells was analyzed by western blot assay after the cells were treated with TVA. Protein expression was quantified by normalization to the level of β-tubulin. c. Effects of IGF-1 on TVA-induced apoptosis as detected by an annexin V-FITC/PI staining assay. The number of apoptotic cells was determined after treatment with TVA (100 μM) in the presence or absence of IGF-1 (50 ng/mL) for 24 h. The values represent the means±standard deviation of three independent experiments. *P<0.05 and **P<0.01 versus the control group

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Lipids in Health and Disease

ISSN: 1476-511X