Table 2 The primers of qRT-PCR
Gene | Primer sequence (5′ → 3′) |
|---|---|
Human actin-Forward | GAGCACAGAGCCTCGCCTTT |
Human actin-Reverse | GAGCGCGGCGATATCATCA |
Human FoxO1-forward | GGCTGAGGGTTAGTGAGCAG |
Human FoxO1-reverse | AAAGGGAGTTGGTGAAAGACA |
Human Pparγ-forward | GGGATCAGCTCCGTGGATCT |
Human Pparγ-reverse | TGCACTTTGGATCTCTTGAAGTT |
Human Adipoq-forward | AACATGCCCATTCGCTTTACC |
Human Adipoq-reverse | TAGGCAAAGTAGTACAGCCCA |
Human Lpin1-forward | GCTGGAGAGGGAGAGAGGAT |
Human Lpin1-reverse | GGTGGCTGCTGTTAGGACA |
Human Acc-forward | TCCGCACTGACTGTAACCAC |
Human Acc-reverse | GCGACTTCCATACCGCATTA |
Human Glut4-forward | TGGGCGGCATGATTTCCTC |
Human Glut4-reverse | GCCAGGACATTGTTGACCAG |
Rattus actin-Forward | AGCCATGTACGTAGCCATCC |
Rattus actin-Reverse | ACCCTCATAGATGGGCACAG |
Rattus Acc-forward | TACAACGCAGGCATCAGAAG |
Rattus Acc-reverse | TGTGCTGCAGGAAGATTGAC |
Rattus APN-forward | AATCCTGCCCAGTCATGAAG |
Rattus APN-reverse | TCTCCAGGAGTGCCATCTCT |
Rattus Lpin1-forward | CCATTCACAGCGAGTCTTCA |
Rattus Lpin1-reverse | TGGAAGGGGAATCTGACTTG |
Rattus Glut4-forward | GCTTCTGTTGCCCTTCTGTC |
Rattus Glut4-reverse | TGGACGCTCTCTTTCCAACT |