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Fig. 3 | Lipids in Health and Disease

Fig. 3

From: Generation of hyperlipidemic rabbit models using multiple sgRNAs targeted CRISPR/Cas9 gene editing system

Fig. 3

Analysis of plasma lipids and lipoproteins. (a) Agarose gel electrophoresis of plasma lipoproteins. 4 μl of plasma was loaded in each well and fractionated on a 1% agarose gel and stained with Fat Red 7B for neutral lipids. Lipoprotein migration positions are indicated by arrows. (b) Analysis of plasma apolipoproteins by Western blotting. Plasma samples (0.5 μl) were fractionated on 10% SDS-PAGE and transferred to a cellulose membrane probed with Abs against apoB, apoE and apoAI as described in the Materials and Methods section. (c) Plasma levels of total cholesterol (TC), triglycerides (TG), LDL-cholesterol (LDL-C) and HDL-cholesterol (HDL-C)

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Lipids in Health and Disease

ISSN: 1476-511X