Table 2 Summary of gene targeting efficiency
Microinjected sgRNAs | sgRNA 2,4,5 | sgRNA 1,3,6 | sgRNA 1,2,3,4 | Total |
|---|---|---|---|---|
No. of embryos collected | 113 | 137 | 179 | 429 |
No. of embryos injected | 96 | 109 | 140 | 345 |
No. of recipients | 5 | 5 | 6 | 16 |
No. of gestations | 3 | 3 | 4 | 10 |
No. of pups born | 10 | 3 | 5 | 18 |
No. of live pups | 4 | 2 | 1 | 7 |
No. of mutant pups | 3 | 2 | 1 | 6 |
No. of pups with large fragment deletion | 1 | 0 | 1 | 2 |
Rate of pregnancy | 60.0%(3/5) | 60.0%(3/5) | 66.7%(4/6) | 62.5%(10/16) |
Rate of mutations | 75.0%(3/4) | 100.0%(2/2) | 100.0%(1/1) | 85.7%(6/7) |