Table 1 Primer used for qPCR
Gene | Accession number | Nucleotide sequence(from 5′ to 3′) | Amplicon(bp) |
|---|---|---|---|
SREBP-1c | NM-001005291 | F: CACTGGTGGTAGATGCGGAGAA R:TCATTGATGGAGGAGCGGTAGC | 139 |
FAS | NM-000043 | F: AGGACATGGCTTAGAAGTGGAA, R: CTTGGTGTTGCTGGTGAGTG | 166 |
SCD1 | NM-005063 | F: CCTGGTTTCACTTGGAGCTGTG R: TGTGGTGAAGTTGATGTGCCAGC | 106 |
CPT-1 | NM-001876.3 | F:TGAGCACGGCAAGATGAGTC R:GAGGCAGCGATGTCTGGAAT | 104 |
PEPCK | NM-002591.3 | F: TGAAAGGCCTGGGGCACAT R: TTGCTTCAAGGCAAGGATCTCT | 155 |
G6PC | NM-000151.3 | F: TCATCTTGGTGTCCGTGATCG R: TTTATCAGGGGCACGGAAGTG | 220 |
18S | NR-003286 | F: CCTGGATACCGCAGCTAGGA R: GCGGCGCAATACGAATGCCCC | 112 |