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Fig. 1 | Lipids in Health and Disease

Fig. 1

From: Active compound chrysophanol of Cassia tora seeds suppresses heat-induced lipogenesis via inactivation of JNK/p38 MAPK signaling in human sebocytes

Fig. 1

PPARγ and FAS are upregulated by heat in human sebocytes. Human sebocytes were serum-starved for 6 h and then incubated for 30 min at 41 °C or 44 °C. Fresh culture medium was added and the cells were cultured. a Cell viability was assessed by MTT assay. b, c PPARγ, FAS and target gene of PPARγ protein levels were analyzed by western blotting, with actin used for normalization. d Intracellular lipids in human sebocytes exposed to heat were assessed by Nile Red staining. Scale bar = 20 μm. Quantitation of lipid levels in human sebocytes exposed to heat, calculated as percentage of the value in cells incubated at 37 °C. Data represent the mean ± SE (n = 3). *P < 0.05, (Student’s t test)

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