Fig. 2

Inhibition of lipid synthesis by CTSE and CP in heat-treated sebocytes. Human sebocytes were serum-starved for 6 h and then incubated for 30 min at 44 °C. Fresh culture medium was added with various concentrations of CTSE (0–100 ppm) or CP (0–100 μM), followed by incubation for 48 h. All experiments included non-heat-shocked and untreated sebocytes as a control. a Cells were treated with various concentrations of CTSE (0–100 ppm) and CP (0–100 μM), and cell survival was analyzed using the MTT reduction assay. Data represent the mean ± SD of triplicate samples expressed as a percentage of the control. b, c Cell lysates were analyzed by western blotting for PPARγ and FAS with actin used for normalization. d Levels of intracellular lipids assessed by Nile Red staining. Scale bar = 20 μm. Lipid levels in heat-shocked and CTSE or CP-treated human sebocytes, calculated as a percentage of the value in cells incubated at 37 °C. Data represent the mean ± SE (n = 3). *P < 0.05 vs. cells incubated at 37 °C; ^†P < 0.05, vs. cells incubated at 44 °C, (Student’s t test)