Fig. 1

FoxO3 expression is elevated in the liver of NAFLD models and patients with NAFLD. a and b Protein and mRNA levels of FoxO3 were analyzed in the livers of mice fed a high fat and high sucrose diet (HFHS) or chow diet for 12 weeks. c and d Protein and mRNA levels of FoxO3 in the livers of ob/ob mice and wild-type (WT) mice fed a chow diet for 12 weeks. e and f Protein and mRNA levels of FoxO3 in the livers of db/db mice and WT mice fed a chow diet for 12 weeks. g The protein level of FoxO3 was measured in the livers of patients with NAFLD and healthy subjects (NC). h Protein analysis of FoxO3 in HepG2 cells exposed to high glucose and high palmitic acid (PA) for 24 h. The data are presented as the mean ± SEM, n = 3–5 for each group. ^*P < 0.05 versus control, ^**P < 0.01 versus control