Fig. 2

Measurement of IL-1 β and IL-6 in lung tissue and bronchoalveolar lavage fluid (BALF) of the ALI rats preconditioned with different lipid emulsions. Rats were preconditioned with different lipid emulsions for one week, and BALF supernatants and lung tissues were collected 8 h after ALI induction from LPS challenge. a, b, qRT-PCR test of IL-1β in lung tissue, band intensity (means for lanes 1–5, 0.111 ± 0.023; 0.435 ± 0.070; 0.592 ± 0.060; 0.214 ± 0.046; and 0.256 ± 0.060 for groups Saline, LPS, Intralipid, Clinoleic, Omegaven, respectively) was quantified with software; c, ELISA test of IL-1β in BALF. d, e, qRT-PCR test of IL-6 in lung tissue, band intensity (means for lanes 1–5, 0.023 ± 0.010; 0.214 ± 0.043; 0.323 ± 0.056; 0.183 ± 0.020; 0.151 ± 0.015 for groups Saline, LPS, Intralipid, Clinoleic, Omegaven, respectively) was quantified with software; f, ELISA test of IL-6 in BALF. g, Measurement of TNF-α with ELISA in bronchoalveolar lavage fluid (BALF). Results were expressed as the means ± SD from the PCR band quantification or the corresponding protein level relative to ELISA reading (number of rats per group, Saline 16, LPS 17, Intralipid 15, Clinoleic 16, and Omegaven 16). *P < 0.05 (ANOVA)