Fig. 1

SULT2B1-deficient mice were susceptible to gastric tumors upon induction with the carcinogenic agent 3-MCA. a SULT2B1 expression in human normal gastric mucosal tissue was studied by immunohistochemical staining and Western blotting analysis. b The SULT2B1 mRNA level in different murine organs was detected by qRT-PCR. c The SULT2B1 mRNA expression level in the gastric tissues of WT and SULT2B1^−/− mice was measured by qRT-PCR. The concentration of cholesterol sulfate in the gastric tissues of WT and SULT2B1^−/− mice was detected by LC-MS. d The gastric carcinoma model was established by 3-MCA induction in WT and SULT2B1^−/− mice. e Three months after induction, the number of tumor-bearing mice with or without bloody ascites in the WT and SULT2B1^−/− mice was plotted. f The survival probabilities of WT and SULT2B1^−/− mice upon 3-MCA treatment. g The results from the H&E staining and immunohistochemical staining for CK8, CKpan, and VIM expression in the nodules are presented. * P < 0.05, ** P < 0.01