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Table 2 Summary of analysis of various anti-oxidants in RIN5F treated with Alloxan(Pre-treatment)/STZ (Sim-treatment) and sub-optimal doses of AA (10 μg/ml) and BDNF (50 ng/ml)

From: PUFAs, BDNF and lipoxin A4 inhibit chemical-induced cytotoxicity of RIN5F cells in vitro and streptozotocin-induced type 2 diabetes mellitus in vivo

SUMMARY OF THE ANALYSIS OF VARIOUS ANTI-OXIDANT ENZYMES IN RIN5F CELLS TREATED WITH AL/STZ
CHEMICALGROUPSOD (Units/mg protein)CAT (Mm H2O2/min/mg proteinGST (mM/min/gm protein)GPX (mM/min/gm protein)
ALLOXANCONTROL23.6 ± 1.7466 ± 52.154.75 ± 1.4739.8 ± 37.2
AL(6 mM)39.88 ± 3.5§739.1 ± 45§56.85 ± 3.2§1649 ± 81.6§
AA(10 μg/ml)19.21 ± 0.9407.7 ± 36.547.74 ± 1.8534.79 ± 24.4
BDNF(50 ng/ml)19.12 ± 1.4384.4 ± 30.941.44 ± 2.2556 ± 18.7
AA + BDNF21.58 ± 2.6326.7 ± 24.938.79 ± 2.1560 ± 16.6
AL + AA(10 μg/ml)21.28 ± 2.8356 ± 6744 ± 0.86*495.3 ± 7.4
AL + BDNF26 ± 1.43#459 ± 76.2#45.5 ± 0.8#503.6 ± 3.5#
AL + AA + BDNF29.25 ± 0.7432.3 ± 6054.4 ± 2.23768 ± 23.6
STREPTOZOTOCINCONTROL24 ± 1.6503.9 ± 45.254.5 ± 0.1701 ± 19.4
STZ(20 mM)39.76 ± 1.4§791 ± 86.1§78.5 ± 0.76 §1036 ± 38.3§
AA(10 μg/ml)21 ± 1.2446 ± 49.351.9 ± 0.2584 ± 23.1
BDNF(50 ng/ml)20.6 ± 1.3420 ± 41.845.5 ± 0.42581 ± 30.1
AA + BDNF24.1 ± 3.1366 ± 32.243.7 ± 0.16550 ± 38.6
STZ + AA(10 μg/ml)25 ± 7.8528 ± 6748.2 ± 0.96505.3 ± 7.4
STZ + BDNF32 ± 1.7#559 ± 86.2#50.5 ± 0.2#603.6 ± 3.5#
STZ + AA + BDNF34 ± 1.08656 ± 57.154.5 ± 0.08752.1 ± 21.4
  1. Superoxide dismutase (SOD) is expressed as U SOD/mg of protein; Catalase (CAT) is expressed as μM of H2O2 consumed/minute/mg of protein; Glutathione-S-transferase (GST) is expressed as μM conjugate formed/ minute/gm of protein; Glutathione peroxidase (GPX) is expressed as μg of glutathione consumed/minute/gm of protein. ₰P < 0.001, § P ≤ 0.01 Vs Untreated control and * P < 0.01,# P ≤ 0.05, ** P < 0.001 Vs AL/STZ -treated group