Table 2 Summary of analysis of various anti-oxidants in RIN5F treated with Alloxan(Pre-treatment)/STZ (Sim-treatment) and sub-optimal doses of AA (10 μg/ml) and BDNF (50 ng/ml)
SUMMARY OF THE ANALYSIS OF VARIOUS ANTI-OXIDANT ENZYMES IN RIN5F CELLS TREATED WITH AL/STZ | |||||
|---|---|---|---|---|---|
CHEMICAL | GROUP | SOD (Units/mg protein) | CAT (Mm H2O2/min/mg protein | GST (mM/min/gm protein) | GPX (mM/min/gm protein) |
ALLOXAN | CONTROL | 23.6 ± 1.7 | 466 ± 52.1 | 54.75 ± 1.4 | 739.8 ± 37.2 |
AL(6 mM) | 39.88 ± 3.5§ | 739.1 ± 45§ | 56.85 ± 3.2§ | 1649 ± 81.6§ | |
AA(10 μg/ml) | 19.21 ± 0.9 | 407.7 ± 36.5 | 47.74 ± 1.8 | 534.79 ± 24.4 | |
BDNF(50 ng/ml) | 19.12 ± 1.4 | 384.4 ± 30.9 | 41.44 ± 2.2 | 556 ± 18.7 | |
AA + BDNF | 21.58 ± 2.6 | 326.7 ± 24.9 | 38.79 ± 2.1 | 560 ± 16.6 | |
AL + AA(10 μg/ml) | 21.28 ± 2.8 | 356 ± 67 | 44 ± 0.86* | 495.3 ± 7.4 | |
AL + BDNF | 26 ± 1.43# | 459 ± 76.2# | 45.5 ± 0.8# | 503.6 ± 3.5# | |
AL + AA + BDNF | 29.25 ± 0.7 | 432.3 ± 60 | 54.4 ± 2.23 | 768 ± 23.6 | |
STREPTOZOTOCIN | CONTROL | 24 ± 1.6 | 503.9 ± 45.2 | 54.5 ± 0.1 | 701 ± 19.4 |
STZ(20 mM) | 39.76 ± 1.4§ | 791 ± 86.1§ | 78.5 ± 0.76 § | 1036 ± 38.3§ | |
AA(10 μg/ml) | 21 ± 1.2 | 446 ± 49.3 | 51.9 ± 0.2 | 584 ± 23.1 | |
BDNF(50 ng/ml) | 20.6 ± 1.3 | 420 ± 41.8 | 45.5 ± 0.42 | 581 ± 30.1 | |
AA + BDNF | 24.1 ± 3.1 | 366 ± 32.2 | 43.7 ± 0.16 | 550 ± 38.6 | |
STZ + AA(10 μg/ml) | 25 ± 7.8 | 528 ± 67 | 48.2 ± 0.96 | 505.3 ± 7.4 | |
STZ + BDNF | 32 ± 1.7# | 559 ± 86.2# | 50.5 ± 0.2# | 603.6 ± 3.5# | |
STZ + AA + BDNF | 34 ± 1.08 | 656 ± 57.1 | 54.5 ± 0.08 | 752.1 ± 21.4 | |