Fig. 2

Time-dependent progression of hepatic NPC1L1-mediated steatosis in L1-Tg mice. a Photographic images of livers from wild-type (WT) and L1-Tg mice fed a high-fat diet (HFD) at independent period. b Time-dependent changes in hepatic levels of cholesterol and triglyceride (TG). To visualize association between cholesterol and TG levels in individual mouse livers, a scatter diagram showing hepatic cholesterol levels on the X axis and TG levels on the Y axis was created for each group of L1-Tg mice fed a HFD for the indicated time periods (w, weeks). All data in the left panel were summarized in the right panel as the mean ± SEM; where bars are not shown, the SEM is within the limits of the closed symbols. The data from the 6 w group were derived from our previous study [11] under a CC-BY license. c Time-dependent changes in the ratios of liver weight to body weight (L/B ratios) in L1-Tg mice fed a HFD for the indicated number of weeks. Data are expressed as the mean ± SEM. n = 6, 8, 6, 12, 7 (0, 1, 2, 3, 6 w). Statistical analyses for significant differences were performed using Bartlett’s test, followed by a non-parametric Shirley–Williams’s multiple-comparison test (**, P < 0.01 vs. 0 w control). The values of mean and SEM are summarized in Additional file 1: Appendix