Fig. 3
From: MiR-9-5p promotes rabbit preadipocyte differentiation by suppressing leptin gene expression
Inhibition of miR-9-5p inhibited rabbit preadipocyte differentiation (a) MiR-9-5p expression levels during preadipocyte differentiation after transfecting with the miR-9-5p inhibitor and INC, (b) Oil Red-O staining of lipid droplets on day 9; (c) Quantitative detection of oil red O staining on day 9; (d) Accumulation of triacylglycerol on day 9; (e) PPARγ expression levels during preadipocyte differentiation after transfecting with the miR-9-5p inhibitor and INC; (f) CEBPα expression levels during preadipocyte differentiation after transfecting with the miR-9-5p inhibitor and INC; (g) FABP4 expression levels during preadipocyte differentiation after transfecting with the miR-9-5p inhibitor and INC (h, i) PPARγ protein levels during preadipocyte differentiation after transfecting with the miR-9-5p inhibitor and INC (“*”, P ≤ 0.05; “**”, P ≤ 0.01)