Table 1 Primers used for real-time quantitative polymerase chain reaction (RT-QPCR) in myocardial tissue from Psammomys obesus gerbils
From: High fat diet altered cardiac metabolic gene profile in Psammomys obesus gerbils
Genes | Primer sequences | |
|---|---|---|
Solute carrier family 2 members 1 (Slc2a1 or GLUT1) | Sense | GTGGGCCTCTTTGTTAATCG |
Antisense | CATAAGCACGGCAGACACAA | |
Solute carrier family 2 members 4 (Slc2a4 or GLUT4) | Sense | GAGTTAGCTGGGGTGGAACA |
Antisense | ACCGAGACCAACGTGAAGAC | |
Carnitine palmitoyltransferase-1B (CPT1B) | Sense | AAGAACACGAGCCAACAAGC |
Antisense | ACCATACCCAGTGCCATCAC | |
Fatty acid binding protein 3 (FABP3) | Sense | TCAAGTCGGTCGTGACACTG |
Antisense | TCCCATCACTTAGTTCCCGTG | |
Fatty acid translocase (CD36) | Sense | TTCAAGGTGTGCTCAACAGC |
Antisense | ACCCCACAAGAGTTCCTTCA | |
AMP-activated protein kinase (AMPK) | Sense | TTCGGGAAAGTGAAGGTGGG |
Antisense | TCTCTGCGGATTTTCCCGAC | |
Pyruvate dehydrogenase kinase 4 (PDK4) | Sense | TTCCAGGCCAACCAATCCAC |
Antisense | TGGCCCTCATGGCATTCTTG | |
Uncoupling protein 3 (UCP3) | Sense | CGCCAGATGAGTTTTGCCTC |
Antisense | CTGGAGTGGTCCGTTCCTTT | |
Peroxisome proliferator activated receptor-alpha (PPAR-α) | Sense | TTAGAGGCGAGCCAAGACTG |
Antisense | CAGAGCACCAATCTGTGATGA | |
Peroxisome proliferator activated receptor-gamma (PPAR-γ) | Sense | GCGCTAAATTCATCTTAACTCCCA |
Antisense | CTGTGTCAACCATGGTAATTTCAGT | |
Insulin receptor substrate 1 (IRS1) | Sense | ATGAACATCAGACGCTGTGG |
Antisense | TCATCCACTTGCATCCAGAA | |
Insulin receptor substrate 2 (IRS2) | Sense | CGGATTTTGGAAGAGGAGAGA |
Antisense | GAGTGATGAGGCTGGGTATGA | |
Natriuretic peptide B (NPPB) | Sense | GACGGGCTGAGGTTGTTTTA |
Antisense | ACTTGAGAGGTGGTCCCAGA | |
Hypoxanthine phosphoribosyl transferase-1 (HPRT1) | Sense | ACAGGCCAGACTTTGTTGGA |
Antisense | TCCACTTTCGCTGATGACCAC |