Fig. 5

Phospholipid analysis of the PI, PS, PA and Cer content of the PLB_WT and PLB4 brain. Analysis of phosphatidylinositols (PI), phosphatidylserines (PS), phosphatidic acids (PA) and ceramides (Cer) in the PLB_WT and PLB4 cortex, hippocampus, and hypothalamus. Each phospholipid class was normalised to its corresponding internal standard. The relative intensity (cps) refers to the normalised total ion count (TIC) for the individual molecular species. Data represented as an average of n = 6. An unpaired, two-tailed Student’s t-test with Welch’s correction was performed to determine statistically significant differences between PLB_WT and PLB4 data; * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001 (white asterisks used only for visibility purposes)