Fig. 3

RNA sequencing analysis of transcriptional changes and GO biological process analysis in cultured LO-2 cells treated with OA (50 μg/mL, 48 h). a After sequencing analysis, the mRNA transcriptional changes between the control and test groups were analyzed. Shown here is a scatter map of mRNA transcriptional changes. b The top 20 upregulated and downregulated genes in the OA treatment group were selected and are shown in a heat map. c RT-PCR was conducted to determine TWIST1, TWIST2 and PPARγ mRNA changes in response to OA treatment as described in the methods section. The results are shown in the bar graphs. d GO biological process analysis was conducted, and the classifications of the upregulated genes are shown. e The classifications of the downregulated genes for GO biological processes are shown